Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses (Nato Science Series C:, 226)

On May 4-8, 1987, a NATO Advanced Research Workshop on the Analytical Uses of Immobilized Biological Compounds was held in Florence, Italy. The Director of the Workshop was Professor George G. Guilbault of the University of New Orleans, and the Co-Director was Professor Marco Mascini of the University of Florence It vas the purpose of this meeting to assemble scientists from all NATO Countries with an interest in immobilized biological compounds. to discuss - methods of immobilization - properties of immobilized compounds - enzyme electrodes and biosensors - optical devices utilizing immobilized enzymes - microbial sensors and clinical uses of immobilized enzymes - flow injection analysis using enzymes - immobilized biological compounds in chemical defense detection - pharmaceutical analysis - uses in industrial analysis - enzyme reactors - air pollution detectors - immunosensors - medical uses and applications - solid state and FET sensors Goals to be achieved by the conference were - to permit an exchange of views and experience in all these areas - to review and critically assess the state-of-the-art in these fields - to set guidelines for future research and establish collaborative projects between scientists in NATO laboratories in the above areas. Thirty-seven lectures were given by 36 speakers in all of the above areas. Front Matter....Pages i-xv Enzymes Immobilized on Inorganic Supports....Pages 1-16 Immobilization of Bioagents by Radiation....Pages 17-23 Structural and Transport Properties of Polymeric Carriers for Bioagents and Immobilization Techniques and their Applications....Pages 25-38 Immobilized Enzymes in Therapy....Pages 39-46 Uses of Immobilized Biological Compounds in Pharmaceutical Analysis....Pages 47-69 Application of Immobilized Enzymes in Diagnostics....Pages 71-82 Immobilized Dehydrogenase Enzymes Coupled with a NADH Sensor....Pages 83-93 Targeting of Immobilized Drugs....Pages 95-113 Enzyme and Microbial Sensor....Pages 115-130 Redox Mediators and Their Application in Amperometric Sensors....Pages 131-140 Uni-, Bi- and Tri-Enzyme Electrodes for Analysis....Pages 141-152 Enzyme Electrochemical Sensors for “In Vivo” Analysis with Artificial Pancreas....Pages 153-167 Biosensors in Clinical and Pharmaceutical Analysis....Pages 169-176 Graft Copolymers as Supports for the Immobilization of Biological Compounds....Pages 177-185 Use of Protein Coatings on Piezoelectric Crystals for Assay of Gaseous Pollutants....Pages 187-194 Use of Immobilized Enzymes in FET-Detectors....Pages 195-206 Micro-Biosensor....Pages 207-218 The Development of Fibre-Optic Sensors by Immobilization of Fluorescent Probes....Pages 219-226 Developing Biosensors....Pages 227-236 Immobilized Biological Compounds in Bio- and Chemiluminescence Assays....Pages 237-248 Immobilized Biospecific Proteins in Analytical Clinical Chemistry....Pages 249-265 Immunosensors....Pages 267-279 Electrochemical Immunosensors....Pages 281-290 Flow Injection Analysis — Recent Develophents and Future Trends....Pages 291-308 Extension to Enzymatic Electrodes of a General Procedure for the Construction of Flow-Through ISEs Based on the use of Conductive Epoxy as Support of the Sensor....Pages 309-317 Biosensor-Based Analyzers: From Design to Instrument....Pages 319-327 Commercial Aspects of the Use of Immobilized Compounds....Pages 329-339 Design Considerations of an Immobilized Enzyme Electrode for Measurement of Glucose in Whole Blood....Pages 341-358 Diffusion Limited Enzyme Electrodes....Pages 359-377 Enzyme Electrodes for Sugar Substitute Aspartame....Pages 379-388 Back Matter....Pages 389-396 Proceedings of the NATO Advanced Research Workshop, Florence, Italy, May 4-8, 1987