Analysis of Marine Samples in Search of Bioactive Compounds (Volume 65) (Comprehensive Analytical Chemistry, Volume 65)

Seas and oceans offer a wide range of temperature, pressure, light and chemical conditions thus allowing a wide diversity of marine organisms from shallow coastal waters to the deep ocean. These resources can be used to obtain new products and develop services, and in turn help to provide solutions to the challenges that affect our planet, including offering a sustainable supply of food and energy, new industrial materials and processes, new bioactive compounds, and new health treatments. Marine compounds have been identified as having antibacterial, anticoagulant, antifungal, antimalarial, antiprotozoal, antituberculosis, and antiviral activities. The major sources of these bioactive compounds are marine sponges, coelenterates, and microorganisms, followed by algae, echinoderms, tunicates, molluscs, and bryozoans. The discovery of bioactive compounds from marine samples is a hot topic considering the current need for sustainable use of marine resources. This book is a comprehensive overview of the analytical techniques employed in the discovery and characterization of bioactive compounds isolated from (all possible) marine samples and gives future perspectives of analytical methodologies. This overview includes an assessment of the sampling and preparation of extracts, the separation and isolation of bioactive compounds, their structural characterization and the application of bioassays in the discovery of bioactive compounds. * Comprehensive coverage of analytical techniques and applications * Clear diagrams to adequately support important topics * Real examples of applications of analytical techniques in the search for new bioactive compounds Content: Front Matter Pages i-iii Copyright Page iv Contributors to Volume 65 Pages xi-xii Series Editor’s Preface Page xiii Preface Pages xv-xvi Chapter 1 - Introduction to the Analysis of Bioactive Compounds in Marine Samples Pages 1-13 Teresa Rocha-Santos, Armando C. Duarte Chapter 2 - Prospection, Collection, and Preservation of Marine Samples Pages 15-34 Sara Leston, Margarida Nunes, João Rosa, Marco F.L. Lemos, Fernando Ramos, Miguel A. Pardal Chapter 3 - Classical Methodologies for Preparation of Extracts and Fractions Pages 35-57 C.I.L. Justino, K. Duarte, A.C. Freitas, Armando C. Duarte, Teresa Rocha-Santos Chapter 4 - Green Analytical Methodologies for Preparation of Extracts and Analysis of Bioactive Compounds Pages 59-78 K. Duarte, C.I.L. Justino, A.M. Gomes, Teresa Rocha-Santos, Armando C. Duarte Chapter 5 - Bioassays for Bioactivity Screening Pages 79-114 Sofia Isabel G.H.M. Montalvão, Vineeta Singh, Shafiul Haque Chapter 6 - Vibrational Spectroscopy for Structural Characterization of Bioactive Compounds Pages 115-148 Keisham S. Singh, Mahesh S. Majik, Supriya Tilvi Chapter 7 - Nuclear Magnetic Resonance Spectroscopy for Structural Characterization of Bioactive Compounds Pages 149-191 Clementina M.M. Santos, Artur M.S. Silva Chapter 8 - Mass Spectrometry for Determination of Bioactive Compounds Pages 193-218 Supriya Tilvi, Mahesh S. Majik, Keisham S. Singh Chapter 9 - Chromatography Coupled to Various Detectors as a Tool for Separation and Determination of Bioactive Compounds Pages 219-252 Regina M.B.O. Duarte, Armando C. Duarte Chapter 10 - Online Combination of Bioassays with Chemical and Structural Characterization for Detection of Bioactive Compounds Pages 253-278 Ana C. Freitas, Sofia Isabel G.H.M. Montalvão, Armando C. Duarte, Teresa Rocha-Santos Index Pages 279-295

• Comprehensive coverage of analytical techniques and applications
• Clear diagrams to adequately support important topics
• Real examples of applications of analytical techniques in the search for new bioactive compounds