Advances in Tracer Methodology : Volume 3 A Collection of Papers Presented at the Ninth and Tenth Symposia on Tracer Methodology

Radioactive tracers have had a profound impact on scientific research during the past twenty years, particularly in bio­ chemistry and allied fields. The rapid evolution of sophisticated instrumentation and labeling techniques during the past ten years has made possible on a routine basis exquisitly sensitive experiments which with other techniques would require her­ culean efforts on the part of the investigator. The Symposia on Advances in Tracer Methodology, which have been held regularly since 1957, provide for research workers in biochemistry, pharmacology, endocrinology, and medicine a focus on the numerous developments in the tracer field that are usually scattered throughout the scientific literature. Volume 3 is arecord of the proceedings of the Ninth and Tenth Symposia, which were sponsored by the New England Nuclear Corporation and the Packard Instrument Company, Inc. Recent advances in tracer methodology, as reflected by the papers in this volume, have been mainly concerned with im­ proved analytical techniques, such as liquid scintillation count­ ing, autoradiography, and chromatography, and with novel ap­ plications of labeled compounds in biochemical and clinical problems. Front Matter....Pages i-x Carbon—14- and Tritium-Labeled DPN and TPN....Pages 1-12 Synthesis and Metabolism of Glucose-3-H 3 and Glucose-4-H 3 ....Pages 13-21 Use of Vinyl Magnesium Bromide in the Synthesis of Labeled Compounds: New Preparations of Glycerol-1-C 14 and Glyceric Acid-1-C 14 ....Pages 23-30 C 14 -Labeled Cardiac Glycosides....Pages 31-36 A Simple Method of Preparing Labeled Benzene....Pages 37-44 Synthesis of 35 S-p-Toluene Sulfonic Anhydride (Tosan) of High Specific Activity....Pages 45-55 The Correlation between Structure and Tritium Incorporation During Labeling by the Wilzbach Method....Pages 57-59 Synthesis of 32 P-, 14 C-, and 3 H-Labeled Lecithins and Their Use in Studies on Lipid Metabolism....Pages 61-67 Improved Methods for Liquid Scintillation Assay of (A) C 14 -Compounds on Paper Chromatograms and (B) C 14 -Protein....Pages 69-79 Quenching Correction in Liquid Scintillation Counting....Pages 81-94 Quench Monitoring and Efficiency Calibration Through External Standardization....Pages 95-106 Comparison of Various Procedures for the Simultaneous Measurement of 32 P and 45 Ca in Inorganic Salts by Liquid Scintillation Counting....Pages 107-117 Problems of Liquid Scintillation Counting on Studies of Metabolic Balances....Pages 119-129 Electron Microscope Autoradiography....Pages 131-137 A New Method of Macroautoradiography....Pages 139-147 Thin Layer Fluorography of Tritium-Labeled Compounds....Pages 149-155 Thin Layer Autoradiographic Electrophoresis of Tritium-Labeled Compounds in Buffered Photographic Gelatin....Pages 157-159 Concentration Profile in Paper Chromatography Investigations of a Labeled Substance....Pages 161-172 The Importance of Isotope Fractionation in the Clinical Application of Labeled Aldosterone....Pages 173-179 Recent Investigations on Cholesterol Metabolism: Critical Evaluation of Radiochromatography and Estimation Procedures....Pages 181-198 Isotope Studies on Citrate-Condensing Enzyme....Pages 199-209 Rapid Inhibitor-Induced Changes in the C 14 -Labeled Compounds Formed by the Photosynthetic Carbon Reduction Cycle....Pages 211-221 Application of Double Isotope Labeling to the Study of the Metabolism of Ascites Tumor Cells under the Influence of Glyceraldehyde....Pages 223-231 Nucleic Acid Metabolism during Antibody Synthesis in Vitro....Pages 233-239 The Biosynthesis of Prostaglandin-E 1 Studied with Specifically 3 H-Labeled Precursors....Pages 241-242 The Use of Labeled Substrates in the Study of Isoprenoid Degradation....Pages 243-256 Use of Different Isotopes in the Study of Cell Differentiation....Pages 257-273 Degradation of C 14 -Labeled Thymidine and its Halogenated Analogues in Man....Pages 275-282 Quantitative Measurement of Transplantation Immunity....Pages 283-284 Studies of the Secretion and the Metabolism of Steroid Conjugates: Synthesis, Analysis, and Use of Doubly Labeled Compounds....Pages 285-299 Countercurrent Distribution Combined with Liquid Scintillation Counting of Double-Labeled Water-Soluble Radioactive Material in the Study of the Metabolism of Steroid Conjugates....Pages 301-311 Conjugation (Detoxification) of C 14 -Labeled Cholic Acid in Human Liver Homogenates as a Test of Liver Function....Pages 313-320 Atmospheric Bomb Radiocarbon as a Tracer in Human Beings....Pages 321-329 Back Matter....Pages 331-333 Advances in Tracer Methodology, Volume 2, records the pro­ ceedings of the 6th, 7th, and 8th Symposia on Advances in Tracer Methodology.