Advances In Molecular Biology And Targeted Treatment For Aids (gwumc Department Of Biochemistry And Molecular Biology Annual Spring Symposia)

Since the discovery of HIV-l as the etiologic agent of acquired immunodeficiency syndrome (AIDS) in the early 1980s, remarkable progress has been made in both the basic understanding of the biological processes leading to AIDS and an accelerated effort in finding new treatments. As is often the case in rapidly advancing fields, most of the scientific discussions are best handled in specialized groups. The effort to organize a meeting on advances in molecular biology and targeted treatment for AIDS was an experiment of sorts to gather experts in selected areas of overlapping interests where advances in basic biology and its application in the development of new drugs could be discussed. Of necessity, the scope of the meeting had to be limited to maintain a certain focus. Important areas of rapid development in AIDS research, such as the vaccine development, epidemiology, animal models, etc. , had to be left out for more specialized meetings. The result, from all accounts, appeared to be quite a successful gathering, which provided a forum for informal discussions among scientists from industry and academic institutions. A remarkable feature of the AIDS virus is its genetic complexity and how some of its seemingly "extra genes" manage to regulate the normal functions of the host and most importantly its immune system. Front Matter....Pages i-ix Mechanistic Analysis of HIV-1 Reverse Transcriptase....Pages 1-19 Analyses of HIV Integration Components....Pages 21-26 DNA Cleaving Activity of Purified Human Immunodeficiency Virus Integration Protein....Pages 27-33 A Thermodynamic Analysis of the Binding of Nucleic Acid to HIV-1 Reverse Transcriptase....Pages 35-50 Rous Sarcoma Virus Gag Gene Sequences Affect Viral RNA Splicing and Stability....Pages 51-54 Ribonuclease H and Primer tRNA Binding Activities of HIV Reverse Transcriptase as Therapeutic Targets....Pages 55-68 Human-Specific Factors are Required for Tat-Mediated Trans-Activation of the HIV-1 and HIV-2 LTRs....Pages 69-77 Role of the TAR Element in Regulating HIV Gene Expression....Pages 79-91 Regulation of HIV-1 Gene Expression by the Tat Protein and the TAR Region....Pages 93-105 HIV-1 Tat: A Transcriptional Activator that Recognizes a Structured RNA Target....Pages 107-121 HIV-1 Tat trans -activates in the Absence of its Target....Pages 123-131 Trans -Activation Requires the Binding of the HIV-1 Tat Protein to Tar RNA....Pages 133-143 The HIV-1 Tat Transactivator Contains an Arginine-Glycine-Aspartyl (RGD) Cell Adhesion Site....Pages 145-150 Inhibition of HIV-1 Replication by Mutant Tat Peptides....Pages 151-172 RNA — Protein Interactions Required for Rev Mediated Regulation of HIV Gene Expression....Pages 173-181 A 5’ Splice Site is Essential for REV and REX Regulation of HIV Envelope Protein mRNA Expression....Pages 183-188 Molecular Functional Studies of HIV-1 REV and NEF Proteins....Pages 189-201 Structure and Function of HIV and SIV Nef Proteins....Pages 203-216 Functional Analysis of the HIV-1 Gene Products vif and vpu ....Pages 217-223 Envelope Pseudotypes of HIV and HTLV....Pages 225-233 CD4- Pseudomonas Exotoxin: A Strategy for AIDS Therapy Based on Selective Killing of HIV-Infected Cells....Pages 235-244 Binding of a Reduced-Peptide Inhibitor and a Statine-Containing Inhibitor to the Protease from the Human Immunodeficiency Virus....Pages 245-249 A T Cell Clone Which Reflects the Functional Defects Observed in the T Cells of AIDS Patients....Pages 251-255 Roles of Nucleocapsid Cysteine Arrays in Retroviral Assembly and Replication: Possible Mechanisms in RNA Encapsidation....Pages 257-272 Regulated Proteolytic Processing within Mature Retroviral Capsids....Pages 273-280 Red Blood Cells Exposing CD4, as Competitive Inhibitors of HIV-1 Infection....Pages 281-299 Gene Therapy for AIDS....Pages 301-308 Discovery of a Novel Potent Inhibitor of HIV Infection....Pages 309-313 Antiretroviral Activity of Carbocyclic Nucleosides and Potential Use of Inosinate Dehydrogenase Inhibitors....Pages 315-321 Lipophilic 6-Halo-2′,3′-Dideoxypurine Nucleosides: Potential Antiretroviral Agents Targeting HIV-Associated Neurologic Disorders....Pages 323-333 Antiviral Evaluation of HIV-1 Specific Ribozyme Expressed in CD4+ HeLa Cells....Pages 335-343 Resistance of Influenza a Viruses to Amantadine and Rimantadine....Pages 345-353 Back Matter....Pages 355-365 Proceedings of the Xth International Washington Spring Symposium at the George Washington University in Washington, D.C., held May 15-18, 1990