Advances in lactoferrin research [Proceedings of the Third International Congress on Lactoferrin, held in May 5-9, 1997, at Le Touquet, France

Following the two meetings on Lactoferrin Structure and Function that were held in Honolulu, Hawaii, in 1993 and 1995, the Third International Conference on Lactoferrin Structure and Function was held in Le Touquet, France, and has successfully reinforced and diversified the previously created bridges between biochemists, clinicians, and companies. In fact, scientists, physicians, and people of industry from different domains have brought a wealth of recent information concerning biochemistry and technical advances in the identification of lactoferrin-derived compounds as well as cell biology, molecular biol­ ogy, pathology, and medical applications of lactoferrin and lactoferrin-derived com­ pounds. We were so delighted with the rapid growth of knowledge concerning many biologi­ cal and immunological functions of lactoferrins and the relationships between their struc­ ture and function, we wanted to share our pleasure with the readers interested in this field. The present book. which represents a review of some of the most exciting contributions, is intended to reflect the status of our knowledge and transmit our hopes for the future devel­ opment of in vivo applications of natural and recombinant lactoferrins. We would like to express our gratitude to the sponsors who contributed to the or­ ganization of the meeting in such a pleasant place and allowed the participation of several young researchers. We would also like to thank all the participants who have answered with enthusiasm our invitation and to every one of the Laboratoire de Chimie Biologique for the constant and efficient help. Front Matter....Pages i-xiii Three-Dimensional Structure of Lactoferrin....Pages 1-14 Structures of Buffalo and Mare Lactoferrins....Pages 15-21 Direct Detection and Quantitative Determination of Bovine Lactoferricin and Lactoferrin Fragments in Human Gastric Contents by Affinity Mass Spectrometry....Pages 23-32 Analysis of Bovine Lactoferrin in Whey Using Capillary Electrophoresis (CE) and Micellar Electrokinetic Chromatography (MEKC)....Pages 33-39 Structural and Immunochemical Studies on Bovine Lactoferrin Fragments....Pages 41-48 Role of the First N-Terminal Basic Cluster of Human Lactoferrin (R 2 R 3 R 4 R 5 ) in the Interactions with the Jurkat Human Lymphoblastic T-Cells....Pages 49-55 Glycation Ligand Binding Motif in Lactoferrin....Pages 57-63 Mouse Lactoferrin Gene....Pages 65-78 Cloning of Human Genomic Lactoferrin Sequence and Expression in the Mammary Glands of Transgenic Animals....Pages 79-83 Expression of Human Lactoferrin in Transfected Rat Mammary Epithelial Cells....Pages 85-89 Restricted Spatiotemporal Expression of Lactoferrin during Murine Embryogenesis....Pages 91-100 Construction of Recombinant Chimeric Human Lactoferrin/Bovine Transferrins....Pages 101-106 The LDL-Receptor Family....Pages 107-111 Identification and Analysis of a Ca 2+ -Dependent Lactoferrin Receptor in Rat Liver....Pages 113-121 Bacterial Lactoferrin Receptors....Pages 123-133 Evidence for the Existence of a Surface Receptor(s) for Ferriclactoferrin and Ferrictransferrin Associated with the Plasma Membrane of the Protozoan Parasite Leishmania donovani ....Pages 135-140 Lactoferrin Secretion into Mouse Milk....Pages 141-153 Lactoferrin in Duodenal Aspirates during Childhood....Pages 155-159 Iron in Synovial Fluid....Pages 161-165 The Gut....Pages 167-173 The Antibacterial Activity of Lactoferrin and Neonatal E. coli Infections....Pages 175-188 Host Defensive Effects of Orally Administered Bovine Lactoferrin....Pages 189-197 Antiviral Activity of Lactoferrin....Pages 199-203 Lactoferrin....Pages 205-213 A Helical Region on Human Lactoferrin....Pages 215-220 Interaction of Lactoferrin with Micrococcus spp . and Its Role in Antimicrobial Activity....Pages 221-228 Enhanced Anti- Candida Activity of Neutrophils and Azole Antifungal Agents in the Presence of Lactoferrin-Related Compounds....Pages 229-237 The Mechanism of in Vivo Bacteriostasis of Bovine Lactoferrin....Pages 239-246 The Main Systemic, Highly Effective, and Quickly Acting Antimicrobial Mechanisms Generated by Lactoferrin in Mammals in Vivo ....Pages 247-250 Regulation by Lactoferrin of Epidermal Langerhans Cell Migration....Pages 251-255 Lactoferrin Stimulates the Mitogen-Activated Protein Kinase in the Human Lymphoblastic T Jurkat Cell Line....Pages 257-260 Effects of Orally Administered Bovine Lactoferrin on the Immune System of Healthy Volunteers....Pages 261-265 Lactoferrin and Interleukin-6 Interaction in Amniotic Infection....Pages 267-271 Inhibition of Azoxymethane Initiated Colon Tumor and Aberrant Crypt Foci Development by Bovine Lactoferrin Administration in F344 Rats....Pages 273-284 Bovine Lactoferrin and Lactoferricin TM Inhibit Tumor Metastasis in Mice....Pages 285-291 Lactoferrin is Synthesized by Mouse Brain Tissue and Its Expression is Enhanced after MPTP Treatment....Pages 293-300 A Study of Lactoferrin and Antibodies Against Lactoferrin in Neurological Diseases....Pages 301-304 Antibodies to Lactoferrin....Pages 305-311 Prevalence and Clinical Significance of Anti-Lactoferrin Autoantibodies in Inflammatory Bowel Diseases and Primary Sclerosing Cholangitis....Pages 313-319 Effect of Lactoferrin on the Phagocytic Activity of Polymorphonuclear Leucocytes Isolated from Blood of Patients with Autoimmune Diseases and Staphylococcus aureus Allergy....Pages 321-330 Lactoferrin....Pages 331-336 Immunohistochemical Demonstration of Lactoferrin in Human Neoplastic Tissues....Pages 337-340 Summary....Pages 341-344 Back Matter....Pages 345-350 Proceedings of the Third International Congress held in Le Touquet, France, May 5-9, 1997