Advances in high pressure bioscience and biotechnology II : proceedings of the 2nd International Conference on High Blood Pressure Bioscience and Biotechnology, Dortmund, September 16-19, 2002

At present, there is a growing interest in high pressure bioscience and biotechnology. The activities are nearly equally distributed between fundamental research and applications. With original work on biochemistry, biophysics, marine and terrestrial micobiology, pharmacology, food science and other applications, this book covers the whole range of current high pressure bioscience. Advances in High Pressure Bioscience and Biotechnology will be welcomed by all academic and industrial researchers who are working in this field. The following topics are covered: High pressure effects on proteins and macromolecules High pressure effects on polyelectrolytes, DNA, RNA High pressure effects on lipids and biomembranes High pressure and enzymatic reactions High pressure microbiology High pressure food science and food technology High pressure applications in bioscience, pharmacy, and medicine High pressure techniques Front Matter....Pages I-XV Front Matter....Pages 1-1 Pressure- and Temperature-induced Phenomena in Proteins....Pages 3-8 High Pressure NMR Spectroscopy Characterizes Higher Energy Conformers of Proteins....Pages 9-14 High Pressure Raman Spectroscopic and RISM Theoretical Studies of Conformational Equilibria of N -Acetyl- L -Glycine- N ’-Methylamide....Pages 15-18 High Pressure as Tool to Study Structural Properties of Thiolate Heme Proteins....Pages 19-24 Essential Differences between the Core and Peripheral Antenna Excitons in Photosynthetic Purple Bacteria Revealed by High-Pressure Spectroscopy....Pages 25-28 Pressure-induced Unfolding of Myoglobin: Neutron Diffraction and Dynamic Scattering Experiments....Pages 29-32 Pressure-induced Critical Association of Myoglobin....Pages 33-36 Fluorescence Spectroscopy of N -acetyl-L-tryptophan-amide and Proteins under High Pressure: Effects of Pressure, Temperature, and Organic Additives....Pages 37-41 Conformational Fluctuations of Hen Lysozyme Investigated by High Pressure NMR Spectroscopy....Pages 43-45 Front Matter....Pages 47-47 Pressure-driven Cold Denaturation of Proteins and Structure Formation of Water-soluble Polymers....Pages 49-54 Exploring the Energy Landscape of Protein Unfolding under High Pressure....Pages 55-59 Double-mutant Thermodynamic Cycles under High Hydrostatic Pressure....Pages 61-68 Pressure Unfolded States of Ribonuclease A under Native and Reducing Conditions have Identical Conformations....Pages 69-72 Hydrostatic Pressure Effects on the Stability of Ataxin-3....Pages 73-77 Insulin and Polylysine as Model Polypeptides for FTIR Studies of the Pressure-effect on Protein Aggregation....Pages 79-82 Kinetic Behaviour of Sulfolobus Solfataricus Carboxypeptidase towards Several Substrates Highlights the Effects of Pressure and Temperature on Non-covalent Interactions....Pages 83-86 The Structural Basis for Functionality and Stability of Hydrophobic Core in RNase A as Probed by the Application of High Pressure....Pages 87-93 Folding and Unfolding Behavior of Carboxypeptidase Y Inhibitor (I C ) Induced by Temperature and Pressure....Pages 95-99 High Pressure NMR Spectroscopy and its Application to the Cold Shock Protein Tm Csp Derived from the Hyperthermophilic Bacterium Thermotoga maritima ....Pages 101-112 Influence of High Pressure on the Secondary Structure of Poly-L-Lysine....Pages 113-116 Front Matter....Pages 47-47 Temperature- and Pressure-induced Unfolding of α-Chymotrypsin....Pages 117-120 Pressure Effects on Ligand Binding Kinetics and the Heme Environment in Myoglobin....Pages 121-124 Front Matter....Pages 125-125 Revisiting some Observations Related to the High Pressure Action on Enzyme Reactions....Pages 127-132 Pressure as a Tool in the Comparative Studies of Fluorescent Proteins....Pages 133-138 Front Matter....Pages 139-139 Interprotein Interactions Perturbed by Pressure. High Pressure Studies on Aggregations of Medical Impact....Pages 141-146 Temperature/Pressure Study of Insulin Fibrillogenesis....Pages 147-150 A High-pressure Approach to Examine Novel Prion Conformational Changes....Pages 151-154 High Pressure Enhances the Chaperone Activity of the Oligomeric Protein Alpha-Crystallin....Pages 155-158 Front Matter....Pages 159-159 New Trends in Macromolecular Crystallography at High Hydrostatic Pressure....Pages 161-170 Effects of Pressure on the Growth Kinetics of Orthorhombic Lysozyme Crystals....Pages 171-174 Effect of Pressure on the Crystallization of Proteins....Pages 175-178 Front Matter....Pages 179-179 Towards an Understanding of the Mechanism of the Aminoacylation Reaction at High Pressure....Pages 181-185 Front Matter....Pages 187-187 Pressure Effects on Columnar Lyotropics: X-ray Diffraction Investigation on d(GMP) and GMP....Pages 189-193 Effects of Cis and Trans Unsaturation on the Barotropic and Thermotropic Phase Behavior of Phospholipid Bilayers....Pages 195-198 Barotropic and Thermotropic Phase Behavior of Bilayer Membranes Composed of Phospholipids Containing Cis Double Bond in the sn -2 Acyl Chain....Pages 199-202 Effects of Gas Pressure on the Phase Transition Temperature of Liposomal Bilayer Membranes....Pages 203-206 High-Pressure Stopped-Flow Studies to Characterize Transient Conformational Transitions of a Membrane Enzyme: Na, K-ATPase....Pages 207-213 Inhibition of Na + , K + -ATPase by Hydrostatic Pressure....Pages 215-219 Hydrostatic Pressure as a Tool to Study F-type ATPsynthases....Pages 221-226 Volume Changes During the Proton Transfer Reactions of Bacteriorhodopsin....Pages 227-230 Front Matter....Pages 187-187 Barotropic Behaviour of Monoolein Cubic Mesophases Embedding Cytochrome c....Pages 231-234 The Role of Matrix Structure in Pressure Regulation of Enzymes Encapsulated in Ternary Surfactant-Water-Organic Solvent Systems....Pages 235-239 Activation of Na + and L-type Ca 2+ Currents in Muscle is More Susceptible to Prolonged High Pressure Treatments than Voltage-dependent Inactivation....Pages 241-246 Front Matter....Pages 247-247 High Pressure Response of Lactic Acid Bacteria....Pages 249-254 Baroprotective Effect of Sucrose and NaCl on Lactococcus lactis ....Pages 255-258 Effects of High Pressure on Bacteria and Fungi....Pages 259-265 The Influence of the Rate of Pressurizing on the Inactivation of Bacillus Subtilis Var. Niger Spores....Pages 267-270 The Role of Tryptophan Permease Tat2 in Cell Growth of Yeast under High-Pressure Condition....Pages 271-274 Low Pressure Shock Response of Yeast....Pages 275-278 Effects of High Pressure and Heat on Muscle Fiber and Connective Tissue of Bovine Skeletal Muscle....Pages 279-282 High Hydrostatic Pressure (HHP) Inactivation of Five Pharmacopoeia Strains Including Spores of Bacillus Subtilis....Pages 283-286 Preliminary Molecular Analysis of a High-Pressure-Modified Type of Bacillus Thuringiensis Ssp. Israelensis , DNA- and Protein-Studies....Pages 287-291 Front Matter....Pages 293-293 High Pressure Applications in Medicine....Pages 295-305 High Pressure Treatment of Human Bonetissue — First Investigations about Mechanics and Biology....Pages 307-309 Apoptosis and Necrosis of Mammalian Cells after High Pressure Treatment — Prospects for a Tumor Vaccine....Pages 311-314 Decontamination and Sterilization of Sensitive Drugs using High Hydrostatic Pressure (HHP)....Pages 315-319 Mechanism of Inactivation of HIV-1 by the Freeze Pressure Generation Method (FPGM)....Pages 321-324 Front Matter....Pages 325-325 High Pressure — Low Temperature Processing of Foods: A Review....Pages 327-340 Influence of High Hydrostatic Pressure on the Formation of Non-enzymatic Browning Products Formed in Maillard-type Reactions....Pages 341-345 Influence of High Hydrostatic Pressure on the Formation of Key Maillard-type Flavour Compounds from D-glucose and L-proline....Pages 347-350 Front Matter....Pages 325-325 Comparison of Structural Cell Membrane Changes Induced by Different High Pressure Processes at Low Temperatures....Pages 351-354 Effects of High-pressure Gas on the Growth Thermograms of Yeast....Pages 355-358 The Influence of High Pressure Treated β-Glucan-gel on the Filterability of Beer....Pages 359-362 Influence of High Pressure Treatment on the Allergenicity of Foods....Pages 363-366 Sterilization by High Hydrostatic Pressure: Increasing Efficiency and Product Quality by Improved Temperature Control....Pages 367-370 High Pressure Freezing Effects on Vegetables....Pages 371-374 Stabilization of Agaricus Bisporus Mushrooms by Combined Action of High Pressure and Heat....Pages 375-378 Effect of High-Pressure on the Denaturation of Meat and Lupin Proteins as Assessed by DSC....Pages 379-384 Transcriptome Profile and Cluster Analysis of Saccharomyces Cerevisiae under Hydrostatic Pressure Conditions....Pages 385-388 High Pressure Inactivation of Yeast and Spheroplasts at Subzero Temperature....Pages 389-393 Transitiometric In Situ Measurements of Pressure Effects on the Phase Transitions During Starch Gelatinization....Pages 395-398 Effect of High Pressures on Enzymatic System of Mesophilic Starters Used for Cheesemaking....Pages 399-402 Stabilisation of Strawberry Puree Treated by High Pressure During Storage....Pages 403-406 Determination of the Activation Parameters of the Early Steps of Greening in Dark-grown Wheat Leaf Homogenates by High Pressure Fluorescence Spectroscopy....Pages 407-412 Biological Sample Preparation System Using Pressure Cycling Technology (PCT)....Pages 413-417 Sterilization of Fruit Juice by High Pressure CO 2 ....Pages 419-424 Freezing Kinetics Due to Ice III Formation in Potato Tissue....Pages 425-429 Effect of Ultra High Pressure Under Argon and Temperature on the Volatiles and Piperine Content and Microbiological Quality of Black Pepper ( Piper Nigrum L.)....Pages 431-436 High Pressure Pasteurisation and Sterilisation of Tomato Puree....Pages 437-440 High Pressure Calorimetry as a Tool to Monitor Phase Transitions in Foods: Application to Water and Selected Lipids....Pages 441-446 Front Matter....Pages 325-325 A Novel High Pressure Technology for the Production of Margarine....Pages 447-451 On the Influence of High Pressure on Edible Oils....Pages 453-457 Pressure Treatment of Food: Instantaneous but not Homogeneous Effect....Pages 459-464 Modelling and Numerical Simulation of Pressure Induced Phase Changes of a Fluid Food System....Pages 465-469 Front Matter....Pages 471-471 High Pressure Differential Scanning Calorimetry of Proteins: Attenuation of Peak....Pages 473-476 Pressure Perturbation Calorimetry....Pages 477-480 On the Calculation of the Compressibility from Ultrasonic Velocity....Pages 481-484 An Internet-based Possibility for Exchange of Informations between High Pressure Researchers — The Ultra High Pressure Webproject....Pages 485-486 Back Matter....Pages 487-490 At present, there is growing interest in high pressure bioscience and biotechnology. The activities are nearly equally distributed between fundamental research and applications. With original work on marine and terrestrial microbiology, biochemistry, molecular biology, deep-sea diving, food science and other industrial applications, this book covers the whole range of current high pressure bioscience. Advances in High Pressure Bioscience and Biotechnology will be welcomed by all industrial and academic researchers who are working in this field.