Advances In Hematopoietic Stem Cell Transplantation And Molecular Therapy (recent Results In Cancer Research)

This book gives an overview of the current state of the art in peripheral blood stem cell transplantation and recent developments in molecular diagnosis and gene therapeutic approaches. The focus is on the role of peripheral blood stem cell transplantation in the treatment of hematological malignancies such as non-Hodgkin lymphomas, chronic myelogenous leukemia and multiple myeloma. Current molecular biological techniques for detecting genetic defects in tumors and minimal residual disease are also presented. Further topics include new gene therapeutic concepts in hematology and oncology: the use of viral vectors for transduction of hematopoietic cells is discussed as well as therapeutic strategies based on antisense nucleic acids, ribozymes, and immunological approaches. Front Matter....Pages I-X Mobilization and Selection of CD 34+ Cells....Pages 1-7 Autologous Stem Cell Transplantation for Chronic Myeloid Leukemia....Pages 8-14 Autologous Stem Cell Transplantation in Aggressive Non-Hodgkin’s Lymphoma....Pages 15-26 Peripheral Blood Progenitor Cell Transplantation in Multiple Myeloma Following High-Dose Melphalan-Based Therapy....Pages 27-35 Molecular Monitoring of Residual Disease in Chronic Myelogenous Leukemia Patients After Therapy....Pages 36-45 Delineation of Genomic Regions in Chromosome Band 7 q22 Commonly Deleted in Myeloid Leukemias....Pages 46-52 Molecular Cytogenetic Analysis of Low-Grade B-Cell Neoplasias: a Comparative Genomic Hybridization Study....Pages 53-60 Applications of Gene Transfer in Hematologic Malignancy....Pages 61-69 Dendritic Cells for Somatic Gene Therapy....Pages 70-77 Construction of Immunogenic Tumor Cell Surfaces by Somatic Gene Transfer....Pages 78-85 Adeno-associated Virus Type 2 Vector for Transduction of Hematopoietic Cells....Pages 86-92 Protection of Hematopoietic Stem Cells from Chemotherapy-Induced Toxicity by Multidrug-Resistance 1 Gene Transfer....Pages 93-115 Regulated Gene Expression After Retroviral Vector-Mediated Delivery of Cancer-Relevant Therapeutic Genes....Pages 116-126 Specific bcr-abl -Directed Antisense Nucleic Acids and Ribozymes: A Tool for the Treatment of Chronic Myelogenous Leukemia?....Pages 127-138 The Use of Ribozymes in Gene Therapy Approaches to AIDS....Pages 139-146 Back Matter....Pages 147-150 Blood stem cell transplantation has become an established therapeutic option in the treatment of malignant diseases. At the same time molecular therapeutic approaches (i.e. gene therapy) are promising alternatives to the classical treatment of cancer. The proceedings give an overview of the biology of hematopoietic progenitor cells, peripheral stem cell transplantation in patients with hematological malignancies, recent developments in molecular diagnosis and gene therapeutic concepts, and show that successful future developments require cooperative research connecting basic science with clinical application.

The book contains black-and-white illustrations.