Advances in Biomedical Photonics and Imaging: Proceedings of the 6th International Conference on Photonics and Imaging in Biology and Medicine (Pibm 2007) Wuhan, P R China, 4-6 November 2007

this Unique Volume Contains Selected Papers Presented At The 6th International Conference On Photonics And Imaging In Biology And Medicine (pibm 2007), Held On November 4-6, 2007 At Wuhan National Laboratory For Optoelectronics, Huazhong University Of Science And Technology, Wuhan, P R China. Pibm Is Designed To Bring Together Scientists, Engineers And Clinical Researchers From A Variety Of Disciplines Engaged In Applying Optical Science, Photonics And Imaging Technologies To Problems In Biology And Medicine. The Scope Of This Conference Ranges From Basic Research To Instrumentation Engineering To Biological And Clinical Studies. It Is Recognized As One Of The Largest And Most Comprehensive International Conferences In China, And Represents The Highest Level Of Worldwide Research In This Field. An Increasing Number Of Young Researchers Are Presenting And Exchanging Their Innovative Ideas On This Friendly And Professional Platform, Thus Making Pibm A Not-to-be-missed Annual Meeting In Wuhan. CONTENTS......Page 12 Preface......Page 6 Organizing Committee......Page 8 Invited Papers......Page 18 1. Introduction......Page 20 1.1. Energy Metabolism and Brain Functions......Page 22 1.2. Spreading Depression and Spreading Depression-like Depolarization......Page 23 1.3. Body Homeostatic Compensatory Mechanisms......Page 24 1.5. Monitoring of Critically Ill Patients in Medical Practice......Page 25 References......Page 26 2. Photosensitizer......Page 30 3. Light Source and Light Delivery......Page 31 4. Tissue Oxygenation......Page 32 5.1. Direct Cytotoxicity......Page 33 5.3. Immune Responses......Page 34 6. PDT Dosimetry......Page 35 7. Future Prospects......Page 36 References......Page 37 2.1. Method......Page 39 3.1. Method......Page 40 4.1. Method......Page 41 References......Page 42 2.1 Photoacoustic Imaging System......Page 44 3. Results......Page 45 References......Page 47 2. Experimental......Page 48 3. Conclusion......Page 49 2. Materials and Methods......Page 50 3. Results and Discussion......Page 52 4. Summary and Conclusions......Page 54 References......Page 55 Topic I – Tissue Optics and Diffuse Optical Imaging......Page 56 1. Introduction......Page 58 3. Results and Discussion......Page 59 4. Conclusions......Page 60 References......Page 61 1. Introduction......Page 62 2. Materials and Methods......Page 63 3. Experimental Results and Discussion......Page 64 4. Conclusions......Page 66 References......Page 67 2.2. Formation and Characteristic of Moire Pattern......Page 68 3.1. Series c sin Expansion Method......Page 69 3.2. Computer Network Algorithm......Page 71 5.3. The Observation Angle of Multi-Direction Moire Deflection System......Page 72 References......Page 73 2. Monte Carlo Simulation Method......Page 74 3. Validation of the Monte Carlo Simulation Program......Page 75 4. The Monte Carlo simulation of polarization imaging......Page 76 5. Conclusion......Page 77 References......Page 78 2. Method......Page 79 3. Simulation......Page 80 5. Conclusion......Page 81 References......Page 82 1. Introduction......Page 83 3. Optical Parameters Measurements and Discussions......Page 84 3.1. Results of the Finite Thickness Homogeneous Slab......Page 85 3.2. Results of the Finite Thickness Two Layered Slab......Page 87 References......Page 88 1. Introduction......Page 89 3. Results and Discussion......Page 90 3.2. The Effect of Mechanical Action......Page 91 References......Page 92 1. Introduction......Page 94 2.3 Procedure......Page 95 3.2 Effect of pH......Page 96 References......Page 97 2.2. Animal......Page 99 3. Result......Page 100 References......Page 101 2.1. Preparation of the Specimens......Page 103 3. Results......Page 104 References......Page 105 2.2. Spectral Measurement......Page 107 3. Results and Discussions......Page 108 4. Conclusions......Page 110 References......Page 111 2.1. The Average Optical Coefficient......Page 112 3.1. The 2-Layered Model......Page 113 4.1. Reconstruction Results......Page 114 5. Conclusion......Page 115 Reference......Page 116 2.1. The Mechanism of Photobleaching......Page 117 3.1. Photon Launching......Page 118 4.2. Porphyrin Bleaching Rate Difference with Different Tumor Optical Parameters......Page 119 References:......Page 120 1. Introduction......Page 122 3. Results and Discussion......Page 123 Acknowledgments......Page 124 References......Page 125 1. Introduction......Page 126 2.4. Data Analyses......Page 127 3.2 Spectral Pre-processing Analyses......Page 128 References......Page 129 1. Introduction......Page 131 2.3. Concentration Measurements......Page 132 3.1 Features of Vis/NIR Spectra and Chemical Structure......Page 133 3.3 Effect of Sensitive Wavelengths......Page 134 4. Conclusions......Page 135 References......Page 136 2. Forward Matrix......Page 137 3. Experiments and Data Processing......Page 138 5.1. Balance of Photon Density by the LSA Method......Page 139 6. Discussions......Page 140 References......Page 141 1. Introduction......Page 143 3. Materials and Methods......Page 144 4. Light Distribution Measurement......Page 145 5. Experimental Results......Page 146 References......Page 147 2. Basic Theory of Fluorescence Analysis......Page 148 4. Research Method and Result Analysis......Page 149 4.2 The Pesticide Residue Analysis of the Vegetable Samples......Page 150 References......Page 152 Topic II – Optical Molecular Imaging......Page 154 2.2. Surface Chemistry of Protein Microarray......Page 156 3. Results and Discussion......Page 157 4. Conclusion......Page 159 References......Page 160 1. Introduction......Page 161 2.4. FRET Image Acquisition and Data Analysis......Page 162 3.2. FRET Analysis of Bid Cleavage Kinetics in NPe6-PDT-induced Apoptosis......Page 163 3.3. Real-time Detection of Bid Redistribution Induced by NPe6-PDT......Page 164 References......Page 165 1. Introduction......Page 167 3.1. Dynamics of Large Near Circular Structure Moving toward Cell Surface after LPLI......Page 168 3.2. Irregular trafficking of GFP after LPLI treatment......Page 169 4. Discussion......Page 170 References......Page 171 2. Materials......Page 173 4.1 Fluorescent Microscope Observation......Page 174 4.3 Variety Relativity Analysis of Biomass in Seawater......Page 175 5.2 Seawater FIM Counting Research......Page 176 Reference......Page 177 1. Introduction......Page 178 2.6. Clonigenic Survival Assay......Page 179 3.3. Clonigenic Survival Assay......Page 180 References......Page 181 1. Introduction......Page 183 2.2. Preparation of Nanofluids......Page 184 3.1. Influence of pH Value on Zeta Potential and Hydrodynamic Size......Page 185 4. Conclusion......Page 186 References......Page 187 2.3. Nanosensor Fabricate......Page 188 2.7. Measurement of Intracellular pH Using Nanosensor......Page 189 Discussions......Page 190 References......Page 191 1. Introduction......Page 192 3.1 Effect of Solvents on the TMP......Page 193 References......Page 194 1. Introduction......Page 195 3. Results and Discussion......Page 196 References......Page 197 1. Introduction......Page 198 2.2.1 HPLC methods......Page 199 2.2.5 Measurement method......Page 200 3.2 Hypoglycemic Function of Insulin-cypate Complex in Diabetic Mouse......Page 201 4 Conclusion......Page 202 References......Page 203 1. Introduction......Page 204 4. Discussion......Page 205 References......Page 206 2.2. Cell Culture and Transient Transfection......Page 207 3.1. Real-time Imaging of dYβC Trafficking from the ER to the Golgi and Plasma Membrane......Page 208 3.2. BACE cleavage of the dYβC probe is initiated in the ER......Page 209 Acknowledgments......Page 210 References......Page 211 2. Experimental Setup......Page 212 3. Results and Discussion......Page 213 References......Page 214 2. Principle of Low Coherence Interferometry......Page 216 3.3 Five-stepping Algorithm (Hariharan Algorithm)......Page 217 4. Result of Numerical Simulation......Page 218 References......Page 221 Topic III – Multiphoton Microscopy in Biomedical Sciences......Page 222 2.1. Preparation of Sample......Page 224 3.1. Characterization of Keratinizing Layer......Page 225 3.3. Characterization of Stroma......Page 226 References......Page 227 Topic IV – Photonic Therapeutics, Diagnostics and Instrumentations......Page 228 2. Methods......Page 230 3. Results......Page 231 4. Discussion......Page 232 References......Page 233 2. Materials and Methods......Page 234 3. Results......Page 235 4. Discussion......Page 237 References......Page 238 2.1. The Multi-Site Multi-Parametric Monitoring System......Page 240 3. Results......Page 241 References......Page 243 1. Introduction......Page 245 2. Method......Page 246 3. Results and Discussion......Page 248 Reference......Page 252 2.2. Hardware......Page 254 2.3. Algorithm......Page 255 Acknowledgments......Page 256 References......Page 257 1. Introduction......Page 258 2.4. Microfluid Chip Isothermal Amplification Detection System......Page 259 3. Results and Discussion......Page 260 References......Page 261 1. Introduction......Page 262 2. Algorithms and Methods......Page 263 References......Page 264 2. Polymorphonuclear Neutrophils......Page 266 3. LIL-rehabilitated Bactericidal Action......Page 267 4. Intravascular Low Energy Laser Irradiation Therapy......Page 268 6. Discussion......Page 269 References......Page 270 2. Laser Beam Intensity Distribution......Page 272 3. Marginal photobiomodulation......Page 273 Acknowledgments......Page 274 References......Page 275 2.1. Materials......Page 276 3. Results and Discussions......Page 277 References......Page 278 1. Introduction......Page 279 2. Rate Equations Model......Page 280 References......Page 284 1. Introduction......Page 285 2.1.2. State equation......Page 286 3. Results and Analysis for Micro-Explosion......Page 287 References......Page 289 2. Principle of PSD......Page 290 3.3. The Method of Neural Network......Page 291 3.4.2 Neural network amendment......Page 292 References......Page 293 1. Introduction......Page 294 2.3 Result & Discussion......Page 295 References......Page 297 2. Neural Network Structure and Methods......Page 298 4.2 Neural Network Prediction Cholesterol Content......Page 299 References......Page 300 1. Introduction......Page 301 3.1. Photo Blanching of HB in PBS Gelatin Solution......Page 302 References......Page 303 1. Introduction......Page 305 3.1. Effects of High Temperature Stress on Photosynthesis Characteristic Parameters in Leaves of Cucumber Seedlings......Page 306 3.3. Effects of high temperature stress on chlorophyll fluorescence parameters in leaves of cucumber seedlings......Page 307 4. Discussion......Page 308 References......Page 309 2. Materials and Methods......Page 311 3.3. The Ultrastructure of Nucleus Pulposus Cells......Page 312 4. Discussions......Page 313 References......Page 314 2. Hardware Design of the System......Page 315 3.2. Theory of Derivative of Zero-Domain (DZD)......Page 316 3.3. Steps of Recognition Method Based on DZD Algorithm......Page 317 References......Page 319 2.1.2 Treatmeat of bacterial species......Page 320 2.1.5 Impedance of E. coli, Salmonellas and Staphylococcus aureus after adding the different antibiotics......Page 321 References......Page 322 2. OCT Image Preprocessing......Page 323 4. Results......Page 324 References......Page 325 2. Material and Methods......Page 326 3. Results......Page 327 4. Discussion......Page 328 References......Page 329 2.1. Experimental Equipment and Materials......Page 331 3.1. The Brain Water Content......Page 332 3.2. The Reduced Scattering Coefficient (μs')......Page 333 4. Discussion......Page 334 References:......Page 335 2. Materials & Methods......Page 337 3.1. Optical Experiments......Page 338 3.2.2. The results of the experiments......Page 339 References......Page 340 2.4 Experimental Procedures......Page 341 4. Discussion......Page 342 References......Page 343 Topic V – Multimodal and Hybrid Biomedical Imaging......Page 344 1. Introduction......Page 346 2. Experimental Systems......Page 347 3. A Reconstruction Algorithm......Page 348 4. Experimental Results and Analyses......Page 349 References......Page 350 1. Introduction......Page 351 2.1. The DR Algorithm......Page 352 2.3. Numerical Implementation......Page 353 3. Results and Discussion......Page 354 References......Page 355 2.1. Imaging Principle......Page 357 3.1. Photoacoustic Imaging Based on the Digital Phased Array System......Page 358 3.3. Photoacoustic Imaging of Breast Tumor by the Digital Phased Array System......Page 359 References......Page 360 1. Introduction......Page 361 2. Analogical Basis Deconstruction (ABD) Theory......Page 362 3. Asymptotic Iterative Estimate (AIE) Algorithm......Page 363 4.1. Simulated Shepp-Logan Experiment......Page 364 References......Page 365 2. Construction of the Medical X-ray Imaging and Methodology......Page 366 4. Medical Application......Page 367 References......Page 368 2.2. The Basic Algorithm Procedure......Page 369 3.2 Parallel Algorithm Procedure......Page 370 5. Conclusions......Page 371 References......Page 372 2.1.4. Entropy the construction of BPA......Page 373 3. The Application of D-S Image Fusion IN The Image Classification......Page 374 References......Page 376 2.1.2. Main reductants......Page 377 2.3.5. Modeling standards......Page 378 2.7. Statistical Method......Page 379 4. Discussion......Page 380 References......Page 381 2. Existence of T-periodic Solutions in D for EP-P Model......Page 382 3. Preliminaries......Page 383 4. Main Results......Page 385 References......Page 386 2.1. Patient Specimen......Page 387 3. Method......Page 388 3.2.1. Image pretreatment......Page 389 4.1. Experiment 1......Page 390 4.2. Experiment 2......Page 391 5.3. Difference with Other Approaches......Page 392 References......Page 393