Adult heart muscle cells : isolation, properties, and applications

The isolation and characterization of calcium-tolerant cells from adult myocardial tissue has proved a major new field of heart research. The wide range of problems which can be examined using single cells in suspension offers the possibility of investigating many important mechanisms underlying diseased states in the myoca rdium. Front Matter....Pages 3-8 Isolated adult heart cells. The development of a new experimental model....Pages 9-12 Ca-tolerant guinea-pig ventricular myocytes as isolated by pronase in the presence of 250 μM free calcium....Pages 13-17 Guinea-pig atrial cardioballs....Pages 19-22 Determination of isolated myocyte viability: Staining methods and functional criteria....Pages 23-29 Sarcolemma-bound calcium. Its importance for cell viability....Pages 31-35 Anoxic injury of adult cardiac myocytes....Pages 37-41 Non-invasive measurements of cell surface receptors....Pages 43-46 Surface antigens of adult heart cells and their use in diagnosis....Pages 47-51 Substrate utilization of adult cardiac myocytes....Pages 53-56 Characteristics of lactate transfer in isolated cardiac myocytes....Pages 57-60 The insulin receptor of adult heart muscle cells....Pages 61-64 Kinetics of cellular uptake of tracers used in myocardial scintigraphy....Pages 65-68 Adhesion of cardiac myocytes to extracellular matrix components....Pages 69-74 Development of new intercellular contacts between adult cardiac myocytes in culture....Pages 75-78 Long-term primary cultures of adult human and rat cardiomyocytes....Pages 79-82 Morphological dedifferentiation of adult cardiac myocytes in coculture with hepatocytes....Pages 83-86 Electrophysiological properties of isolated ventricular myocytes....Pages 87-91 The effect of cyanide on the K-current in guinea-pig ventricular myocytes....Pages 93-96 Measurements of single-channel currents in the membrane of isolated cells: ATP-dependence of K + -channels....Pages 97-99 Evidence for Ca-mediated inactivation of I Ca in dialysed guinea-pig atrial cardioballs....Pages 101-105 Cardiac membrane currents and energetic state....Pages 107-110 Simultaneous measurements of action potentials and contractions in single cultured adult and embryonic heart muscle cells....Pages 111-115 The two components in the shortening of unloaded ventricular myocytes: Their voltage dependence....Pages 117-121 Temperature dependence of verapamil action....Pages 123-127 Microtubules and desmin filaments during the onset of heart growth in the rat....Pages 129-132 The correlation between catecholamine and lipid peroxidation induced damage in heart cells....Pages 133-136 Anoxia in neonatal rat heart cell cultures....Pages 137-142 Enzyme release and glycolytic energy production....Pages 143-147 Anoxia influences the lateral diffusion of a lipid probe in the plasma membrane of isolated cardiac myocytes....Pages 149-152 Summaries of the discussions of the symposium....Pages 153-159 Abstracts of the poster-session on the adult myocyte model....Pages 161-164 Back Matter....Pages 165-166 With this volume results become generally available of a recent conference that was the first to discuss properties of isolated heart muscle cells from the adult myocardium as a new cardio logical research tool. The conference was held as an International Erwin Riesch Symposium un der the auspices of the Deutsche Gesellschaft fUr Herz- und Kreislaufforschung at Spangenberg Castle, March 22-25, 1984. It was made possible through funding by the Erwin Riesch Foun dation and the Bayer AG which deserve our gratitude for their generosity. It may not be fully reflected in this collection of papers that the atmosphere of this meeting was unique. The reason for this resided apparently in the still rudimentary experiences with the adult cardiocyte model that is now widely acknowledged to be very promising. Thus, quite basic inter ests brought together experts from different biological disciplines who in respect to this new mod el were all still "beginners". It was noteworthy that such common interests exerted a unifying effect upon otherwise rather separated fields of science. The main task of the years to come will certainly consist in improving isolation and culturing conditions for adult myocytes in order to fully establish the isolated cell model in heart research