8th International Conference on the Development of Biomedical Engineering in Vietnam : proceedings of BME 8, 2020, Vietnam : healthcare technology for smart city in low- and middle-income countries

This book presents cutting-edge research and developments in the field of biomedical engineering, with a special emphasis on results achieved in Vietnam and neighboring low- and middle-income countries. Covering both fundamental and applied research, and focusing on the theme “Healthcare technology for smart city in low- and middle-income countries,” it reports on the design, fabrication, and application of low-cost and portable medical devices, IoT devices, and telemedicine systems, on improved methods for biological data acquisition and analysis, on nanomaterials for biological applications, and on new achievements in biomechanics, tissue engineering, and regeneration. It describes the developments of molecular and cellular biology techniques, and statistical and computational methods, including artificial intelligence, for biomedical applications, covers key public/occupational health issues and reports on cutting-edge neuroengineering techniques. Gathering the proceedings of the 8th International Conference on The Development of Biomedical Engineering in Vietnam, BME 8, 2020, Vietnam, the book offers important answers to current challenges in the field and a source of inspiration for scientists, engineers, and researchers with various backgrounds working in different research institutes, companies, and countries. Preface Contents Medical Instrumentations Device to Detect Acupuncture Points in the Feet Soles for Massage Treatment 1 Introduction 2 Related Works 3 Hardware Platform 3.1 Sensor Max30100 3.2 Vibration Motor 4 Acupressure Detection Method 4.1 Principle of Measuring SpO2 4.2 Algorithms for Acupuncture Points 5 Experimental Setup and Results 5.1 Experimental Setup 5.2 Accuracy Verification Methods 6 Conclusion References Wireless Heart Rate Monitoring Using Sound Sensor 1 Introduction 2 Background and Related Work 3 A Design for Wireless Heart Rate Monitoring 3.1 Doctor’s Side for Monitoring the Heart Rate 3.2 Patient’s Side with the Sound Sensor 3.3 Wireless RF Module for Communication 4 A Prototype for Heart Rate Monitoring 4.1 The Amplifier and the Sound Sensor 4.2 The MCU 4.3 The RF Module 5 Measurement Method 5.1 Scheme for Measuring the Heart Rate 5.2 Amplifying and Sampling the Sound of the Heart 5.3 Counting and Display the Heartbeat 6 Experimental Results 6.1 Experimental Setup 6.2 Result 7 Conclusion and Future Work References Equipment for Contact-Free Respiratory Measurement and Obstructive Sleep Apnea Detection 1 Introduction 2 Related Work 3 Hardware Platform 4 Respiratory Rate Counting and Sleep Apnea Detecting Method 4.1 Calculating Real Distance Values 4.2 Algorithms for Measuring Respiratory Rate and Detecting Sleep Apnea 5 Experimental Setup and Results 5.1 Experimental Setup 5.2 Results 6 Conclusion References Non-invasive Pressure Support Ventilator for Patients with Respiratory Failure in Under Resourced Regions 1 Introduction 2 General Description of the Device 3 Ventilator Components, Data Processing and Control 3.1 Blower 3.2 Pressure Transducers 3.3 Flow Measurement 3.4 Microcontroller 3.5 Operator Interface 3.6 Ventilator State Control 4 Suitability of the Ventilator for Low Resource Regions 5 Conclusions References Implementation of a Drum Collector for Electrospinning Machines Based on Embedded System 1 Introduction 1.1 The Artificial Approach to Cure Wound Healing 1.2 The Principal of Electrospinning Machines 2 Designing of a Drum Collector for the Electrospinning Machine Mechanical Design 2.1 Mechanical Design 2.2 Hardware Development 3 Methodology 4 Result of Experiment 4.1 Optimized Parameters 4.2 Morphology Analysis 4.3 Membranes Under SEM Image Observation 5 Conclusion References Development of a Low-Cost and Portable Real-Time PCR Machine for Developing Countries 1 Introduction 2 Related Works 3 Materials and Methods 3.1 Thermocycler 3.2 Optical System 3.3 Heat Lid 4 Results and Discussion 4.1 Fluorescence Acquisition System 4.2 Temperature Control 4.3 Optical Setup 4.4 Real-Time PCR 5 Conclusions References Design and Fabrication of a Complete Electrospinning System 1 Introduction 1.1 Some Methods to Treat Wound Healing 1.2 Basic Component of Electrospinning Machines 2 Design an Electrospinning System Consists of a Fume Hood Controller 2.1 Mechanical Design 2.2 Hardware Development 3 Firmware Development 3.1 The Operation of the Control Panel Board 3.2 The Description of How the Main Board Control Operated 3.3 The Control Syringe Pump and Slider Procedures 4 Result of Experiment 4.1 Testing Methodology 5 Conclusion References Design of a Soft Robotic Glove for Hand Rehabilitation Based on Pneumatic Network Method and Low Cost Electro-pneumatic Device 1 Introduction 2 Related Work 3 Methods 3.1 Design Elastomeric Materials 3.2 Electro-pneumatic Design 3.3 Pressure Sensor Calibration 4 Experimental Test and Results 4.1 Electro-pneumatic System 4.2 Force Measurement 4.3 Grasping of Objects of Various Shapes 5 Conclusion and Future Works References Portable Hand Vein Finder System Based on Near-Infrared Illumination and Morphological Image Processing 1 Introduction 2 Materials and Methods 2.1 Principle of Near-Infrared Imaging in Vein Finder 2.2 Main Components of a Portable Vein Finder Prototype 3 Results and Discussion 3.1 Image Processing for Displaying on a Monitor 3.2 Image Processing for Projecting the Venous Patterns on the Skin 3.3 Design of a Portable Vein Finder Prototype 3.4 Portable Vein Finder Prototype 4 Conclusion References Applying Soft Actuator Technology for Hand Rehabilitation 1 Introduction 2 Materials and Design 2.1 Materials 2.2 Soft Pneumatic Actuator 3 Results and Discussion 3.1 Mold Design 3.2 Simulation and Actual Results 3.3 Glove Design for Hand Rehabilitation 4 Conclusion References Design of an Automatic System for Bioelectrical Impedance Phase Angle Measurement Using an Analog Multiplier 1 Introduction 2 Methods and Materials 2.1 Electrode Method 2.2 Power Supply 2.3 Current Source 2.4 Instrumentation Amplifier 2.5 Bioelectrical Impedance Phase Angle Measurement Unit 3 Results and Discussion 3.1 Assessment of the Sine-Wave Generator Using AD9833 3.2 Assessment of the Current Source 3.3 Assessment of the Phase Angle Measuring Unit 4 Conclusion References Smart Blind Stick for Visually Impaired People 1 Introduction 2 Materials and Methods 2.1 Stick Construction 2.2 Obstacle Detection and Alarming System 2.3 Data Transmission 2.4 Power Source 3 Results and Discussions 3.1 Testing Procedure 3.2 Obstacle Detection Experiment 3.3 Data Transmission Experiment 4 Conclusion References Biomechanics A Study of Polishing Parameters to Surface Roughness of Magnetorheological Polishing Methods 1 Introduction 2 Principle of MRP 3 Experimental Setup 4 Results and Discussion 4.1 Effect of Polishing Speed 4.2 Effect of Abrasive Sizes 4.3 Effect of Electric Current 5 Conclusions References Effect of Applied Load and Surface Texture on Friction of UHMWPE in Total Joint Replacement 1 Introduction 2 Materials and Methods 2.1 Preparation of Specimens 2.2 Preparation of 25 mg/ml BSA 2.3 Frictional Testing Apparatus and Procedure 2.4 Data Analyses 3 Results and Discussion 3.1 Effect of Applied Load on Friction of UHMWPE 3.2 Effect of Surface Texture on Friction of UHMWPE 4 Conclusions References An Automatic Control System for Measuring Stokes Polarization and Utilizing UV Light Source 1 Introduction 2 Methods 2.1 Methodology 2.2 Hardware Design 2.3 Firmware and Software Development 3 Results and Discussion 3.1 Designed Hardware 3.2 Validation for Accuracy of the System 4 Conclusion References A Statistical Approach to Evaluate Beta Response in Motor Imagery-Based Brain-Computer Interface 1 Introduction 2 Methodology 2.1 Subjects and Environment 2.2 Data Acquisition 2.3 Experiment Paradigm 3 Data Processing 4 Results and Discussion 4.1 Temporal Relative Power Plot 4.2 Beta Response During Motor Imagery 4.3 Characteristic Frequency Band During Motor Imagery 5 Conclusion References Internet of Things and Telemedicine A New Design of an Automated System to Collect and Monitor Temperature and Humidity 1 Introduction 2 Experimental Design 2.1 System Design 2.2 Hardware Design 2.3 Software Implementation 3 Result and Discussion 3.1 Experimental Result 3.2 Discussion 4 Conclusion References Design of NIBP Holter System Based on NIBP Device and Smartphone 1 Introduction 2 Design System 2.1 Principle Diagram Design 2.2 iChoice BP1 Device 2.3 Design the App for Patient 2.4 Design the App for Doctor 2.5 Design the Database 3 Designing and Testing Results 3.1 Designing Result 3.2 Testing Result 4 Conclusions References Research and Design of Braille Learning Aids for Visually Impaired People Applying IoT Technology 1 Introduction 2 Design System 3 System Modes and IoT Technology Applications 4 Experiment and Evaluate the Results References Internet of Things Application for Online Surveillance and Control of Attacks by Sea Lice 1 Introduction 2 Materials and Methods 2.1 Electronic Module 2.2 Microfluidic Module 2.3 Process Flow 2.4 Water Sample Preparation 3 Results and Discussion 4 Conclusion References Efficiency Evaluation of a Pilot Telemedicine System to Monitor High Blood Pressures in Binh Duong Province (Vietnam) 1 Introduction 2 Materials and Method 2.1 The Tele-blood Pressure Device 2.2 The Cloud Telemedicine Information System (CTIS) 2.3 The Patients Section Process for the Study 2.4 Clinical Monitoring Process 2.5 Users Survey 3 Results 3.1 Characteristics of Participants in 2 Groups 3.2 Comparison of the Results of the Two Research Groups at Each Study Time 3.3 Subclinical Results 3.4 Evaluate the Effectiveness of Health Care in the Performance of the Telemedicine System 4 Conclusions References Pharmaceutical Engineering and Nanomaterials in Medical Applications Improvement of Curcumin Solubility and Bioactivities by the Designed Redox Nanoparticles 1 Introduction 2 Methods 2.1 Synthesis of PEG-siPMNT 2.2 Synthesis of siRNP and Curcumin Encapsulated siRNP (CUR@siRNP) 2.3 Characterization of siRNP 2.4 Investigation of Nanoparticle Bioactivities 2.5 Statistical Analysis 3 Results and Discussion 4 Improvement in the Solubility of Curcumin 4.1 Characterization of siRNP 4.2 The Bioactivities of Curcumin and CUR@siRNP 5 Conclusion References Preparation of Trimethyl Chitosan-Based Polyion Complex Micelle as Drug Delivery System for Cancer Therapy 1 Introduction 2 Materials and Methods 2.1 Synthesis of TMC 2.2 Preparation of TMC/PAAc Micelle and DOX-Loaded TMC/PAAc Micelle 2.3 DOX Release Profile 2.4 Anticancer Activity In Vitro 3 Results and Discussion 4 Conclusion References Enhancing In Vitro Bioavailability of Berberine by Incorporation of Beta–Cyclodextrin Complex into Solid Dispersion System 1 Introduction 2 Materials 3 Preparation and Evaluation 3.1 Preparation and Evaluation of Berberine Complex 3.2 Solid Dispersion Preparation 4 Results 4.1 Solubility of Berberine Delivery System 4.2 Dissolution Profile of Berberine Delivery System 4.3 PXRD of Berberine Delivery System 5 Discussion 6 Conclusion References Solid Lipid Nanoparticles Containing Berberine by Spray-Drying Method 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussion 3.1 Effect of the Preparation Process on SLNs Physicochemical Properties 3.2 Effect of Surfactant on SLNs Physicochemical Properties 3.3 Effect of Spray-Drying Process on Berberine-Loaded SLNs Physicochemical Properties 4 Conclusion References Lipid-Based Vesicles Containing Rutin: Phytosome and Niosome 1 Introduction 1.1 Rutin 1.2 Phytosome and Niosome 1.3 Market Products 1.4 Research Objective 2 Methodology 2.1 Materials 2.2 Preparation of Phytosome and Niosome Loading Rutin 2.3 Evaluation Method 3 Result and Discussion 3.1 Materials 3.2 Evaluate the Stability of Prepared Phytosomes in 30 days (Table 3) 3.3 Effect of Cholesterol on Size, PDI, and Zeta Potential of Niosome 3.4 Effect of Sonication Time on Size, PDI, and Zeta Potential of RP and NP 3.5 Entrapment Efficiency and Loading Capacity 4 Conclusion References Synthesis and Evaluation of Thermoresponsive PLA-PEG-PLA and pH/Temperature-Sensitive OS-PLA-PEG-PLA-OS Hydrogels as Injectable Drug Delivery Application 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussion 3.1 Synthesis and Characterization of PLA-PEG-PLA and OS-PLA-PEG-PLA-OS Copolymers 3.2 Sol–gel Transition Behavior of Block Copolymers 3.3 In Vitro Degradation of Block Copolymers 4 Conclusion References A Colorimetric Aptasensor for Detection of Tetracycline Based on Aggregation of Silver Nanoparticles 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussion 4 Conclusion References Fast Detection of Staphylococcus aureus Using DNA Aptamer and Silver Nanoparticles 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussion 4 Conclusion References Application of Vancomycin Therapeutic Drug Monitoring in a Vietnamese Private Hospital 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussion 4 Conclusion References Tissue Engineering and Regenerative Medicine Fabrication and Characterization of Porous Flow-Assembled Chitosan Membranes in Microfluidics 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussion 4 Conclusion References Fabrication of Virgin Coconut Oil-Loaded Electrospun Polycaprolactone/Polyurethane Membrane for Application in Vascular Engineering 1 Introduction 2 Materials and Methods 2.1 Material 2.2 Methods 2.3 Determination of Clotting Times of Blood Plasma 3 Results and Discussion 3.1 Electrospinning Diameter for PU/PCL Loaded VCO 3.2 Effect of VCO and Electrospinning Parameters on Mechanical Properties of the Membranes 4 Conclusion References Parameters’ Investigation of Tubular Electrospinning System for the Fabrication of Polyurethane (PU)/Polycaprolactone (PCL) Small Hollow Tube for Vascular Engineering’s Applications 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussions 3.1 Tubular Electrospun System 3.2 Effect of Applied Voltage 3.3 Effect of Feeding Rate (Pumping Speed) 3.4 Effect of Collector-Needle Distance 3.5 Effect of Rotation Speed 4 Conclusion References Chitosan Oligomer Mono-coated and Multi-coated Nanofibrous Polycaprolactone Toward the Characterization of Mechanical Strength for Wound Dressing Application 1 Introduction 2 Material and Method 2.1 Materials 2.2 Method 3 Result and Dicussion 3.1 Characterization of OCS 3.2 Morphological and Physicochemical Characterization of PCL.OCS Membrane 4 Conclusion References Effect of In Vitro Formed Fibrillar Fibronectin on Fibroblast Cellular Migration 1 Introduction 2 Material and Method 2.1 Materials 2.2 Methods 3 Results 3.1 Morphologies of FN Fibrils 3.2 Cell Migration 4 Discussion 5 Conclusion References Noninvasive System for Tracking Naïve Induced Pluripotent Stem Cells During Reprogramming 1 Introduction 2 Materials and Methods 2.1 Plasmid Preparation 2.2 mESCs Transfection 2.3 Genotyping Assay 2.4 Differentiation of mESCs 2.5 iPSCs Production 2.6 Quantitative Reverse Transcription (RT)-PCR 3 Results and Discussion 3.1 Generation of Female Dual-Color-Fluorescent-Labeled mESCs 3.2 Live Cell Imaging for Monitoring X Chromosome Status 3.3 Detection of Heterogeneous Population of iPSCs 3.4 High Throughput Screening for Culture Conditions that Promote Acquisition of Pluripotency 4 Conclusion References Fabrication of Injectable Hydrogel from Decellularized Adipose Tissue 1 Introduction 2 Materials and Methods 2.1 Preparation of Decellularized Adipose Tissue (dAT) 2.2 Preparation of Hydrogel from dAT 2.3 Oil Red O Staining 2.4 Hematoxyline and Eosin (H&E) Staining 2.5 DNA Extraction and Quantification 2.6 Scanning Electron Microscope (SEM) Analysis 2.7 In Vitro Cytotoxicity Testing 2.8 Examination of In Vivo Gelation 3 Results 3.1 Preparation of dAT 3.2 Macroscopic and Ultrastructural Evaluation of dAT Hydrogel 3.3 In Vitro Cytotoxicity Testing 3.4 In Vivo Gelation References Effect of the Grafting of Conjugated Linoleic Acid on Crystallinity and Thermal Properties of Electrospun Polymeric Membranes and Its Anticoagulation Mechanism 1 Introduction 2 Materials and Methods 2.1 Fabrication of CLA-Grafted PU/PCL Membrane 2.2 Characterization of Crystallinity and Thermal Properties 2.3 Investigation of Blood-Material Interaction 2.4 Determination of Clotting Times of Blood Plasma 3 Results 3.1 XRD Spectra 3.2 Thermal Behavior of the PU/PCL Membrane and CLA-Grafted Membranes 3.3 Alteration in Clotting Times of Blood Plasma After Exposure to the Membranes 4 Discussion 5 Conclusions References Molecular and Cellular Biology in Medicine Optimization of High Resolution Melting (HRM) Method for Genotyping and Estimating the Frequency of SNP rs751402 on ERCC5 Gene in Vietnamese Breast Cancer Patients 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Result 4 Discussion 5 Conclusion References Purification of Alpha-Fetoprotein from Human Umbilical Cord Plasma 1 Introduction 2 Materials and Methods 2.1 Source of hAFP 2.2 Removal of Albumin 2.3 Isolating hAFP 2.4 Gel Filtration Chromatography 2.5 Native-PAGE 2.6 Western Blotting 2.7 Protein Quantification 3 Results 3.1 Determine the Presence of hAFP in Human Cord Plasma Samples 3.2 Removal of ALB from Human Cord Plasma 3.3 Capturing of hAFP by Avidity Chromatographic Column Using Anti-AFP Rabbit IgG Antibodies 3.4 Purify hAFP by Gel Filtration Chromatography 4 Discussion 5 Conclusions References The Influence of High D-glucose Concentrations on Increasing the Expression of EGR-1, PTEN and GGPS-1 Involved in Insulin Resistance of AT-MSCs 1 Introduction 2 Material and Methods 2.1 Stem Cell Culture 2.2 The Treatment of High D-glucose Concentrations on AT-MSCs 2.3 Quantitative Reverse Transcription Polymerase Chain Reaction 2.4 Western Blotting 2.5 Statistical Analysis 3 Results 3.1 The Effect of High D-glucose Concentrations on the Morphology of AT-MSCs 3.2 The Expression of GGPS-1 in AT-MSCs Under the Influence of High D-glucose Concentrations 3.3 The Expression of PTEN in AT-MSCs Under the Influence of High D-glucose Concentrations 3.4 The Expression of EGR-1 in AT-MSCs Under the Influence of High D-glucose Concentrations 4 Discussion References Antioxidant Effect of Syringic Acid Grafted Chitooligosaccharides in RAW264.7 Cells 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results and Discussion 3.1 Structural Characterization of SA-g-COSs 3.2 Assessment of Cell Viability in RAW264.7 3.3 Reducing Power 3.4 DPPH Free Radical Scavenging Effect 3.5 Hydroxyl Free Radical Scavenging Effect 3.6 Inhibition of Radical Mediated DNA Damage in Raw264.7 3.7 Inhibitory Effect of Membrane Lipid Peroxidation in RAW264.7 4 Conclusion References Identification of Plant Disease Based on Multi-feature Extraction 1 Introduction 2 Proposed Method 2.1 Plant Village Dataset 2.2 Contrast Enhancement 2.3 Gray Level Co-occurrence Matrix 2.4 Local Binary Patterns 2.5 Support Vector Machine 3 Results and Discussions 4 Conclusion References Cyclodipeptides Isolated from a Marine-Derived Fungus Penicillium chrysogenum M612 of Bai Tu Long Sea, Quang Ninh, Vietnam 1 Introduction 2 Material and Methods 2.1 General 2.2 Marine Materials 2.3 Extraction and Isolation 2.4 Physical and 1-D NMR Data of Isolated Compounds (1–7) 3 Results and Discussion 3.1 Structure and Biological Activities of Isolated Compounds (1–7) 4 Conclusion References Evaluation of a Protein-Depletion Kit for Enriching Low Abundant Proteins from Human Sera 1 Introduction 2 Materials and Methods 2.1 Sample Preparations 2.2 Removal of High Abundant Proteins from Serum 2.3 SDS-Page 2.4 Two-Dimensional LC/MS Analysis 2.5 Data Analysis 3 Results and Discussion 3.1 Quantitative Analysis of Serum Protein Samples After Depletion 3.2 Analysis of HAP Depletion Efficacy Using SDS-PAGE 3.3 Identification and Absolute Quantification of Serum Protein After Depletion 4 Conclusion References Ongoing Initiatives in Bringing the Preemptive Panel—Based Pharmacogenetic Testing to Clinical Decisions in Vietnam 1 Introduction 2 Materials and Methods 3 Results and Discussion 4 Conclusion References Optimization of HRM Method for Genotyping and Estimating the Frequency of SNP rs12325489 on Lincrna Gene in Vietnamese Breast Cancer Patients 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Methods 3 Results 3.1 HRM Method Development 3.2 HRM Optimization 3.3 Evaluate the Potential of SNP Rs12325489 4 Discussion 5 Conclusion References The High D-Glucose Concentration Reduces the Ability of Wound Healing in Vitro of Human Adipose Tissue-Derived Mesenchymal Stem Cells 1 Introduction 2 Material and Methods 2.1 Stem Cell Culture 2.2 Cell Proliferation Assay 2.3 Quantitative Reverse Transcription-Polymerase Chain Reaction 2.4 In Vitro Scratch Assay 2.5 Statistical Analysis 3 Results 3.1 The Effect of High D-glucose Concentrations on Growth Ability of AT-MSCs 3.2 The High D-glucose Concentration Decreased Migration Factors Expression in AT-MSCs 3.3 The High D-glucose Concentration Reduced the Ability of AT-MSCs to Heal Chronic Wounds in Vitro 4 Discussion References Evaluate and Optimize Cell-Free RNA Extraction Methods to Apply for Alzheimer’s Disease Biomarkers Detection 1 Introduction 2 Materials and Methods 2.1 Experimental Design 2.2 Plasma Preperation 2.3 RNA Extraction 2.4 PCR and qPCR 2.5 Statistical Analysis 3 Results 3.1 Hemolysis Status 3.2 RNA and ssDNA Yield 3.3 Detection of Housekeeping and Alzheimer’s Disease-Related Transcripts in Plasma Cell-Free cDNA 4 Correlation Between ssDNA and Ct Value 4.1 Operational Factors 5 Discussion 5.1 Hemolysis Status 5.2 RNA and ssDNA Results References Determination of Structure and Anticancer Activity of MM1 Compound Isolated from Endophytic Fungus Aspergillus terreus-RTN3 of Alpinia chinensis Rosc. 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Investigation of Antibacterial, Antifungal Effects of Crude Extracts from A. terreus Culture Solution 2.3 Investigation of Antibacterial and Antifungal Activities of Crude Extracts 2.4 Split the Segments for Antibacterial Activity from the Crude Extract 2.5 Methods for Determining the Chemical Structure of a Compound 2.6 Identification 2.7 Cell Toxicity Test 2.8 Statistical Analysis of Data 3 Results 3.1 Investigation of Antibacterial Activity of Extracts from Fungal and Biomass Media 3.2 Cultivation and Extraction of Antibacterial Active Ingredients from A. terreus R-TN3 Strain 3.3 Locate Antibacterial Active Substance on the Thin Plate 3.4 Refine the Sample by Column Chromatography 3.5 Determination of Antioxidant Ability by DPPH of MM1 Compound 3.6 Survey of Inhibition of Cancer Cell Lines Ability of MM1 Compound 3.7 Mechanism of Inhibiting Cancer Cells of MM1 Compound 3.8 DNA Fragmentation Test Results 3.9 Testing Fluorescence Microscope with AO/EB Dual Dyes 4 Discussion 5 Conclusion References Identification of Bacterial Profile in Root Canals of Teeth with Chronic Periapical Lesions in Vietnam 1 Introduction 2 Materials and Methods 2.1 Patients and Samples Setting 2.2 Sampling Procedure 2.3 Isolation and Evaluation of Bacteria 2.4 PCR Amplification and 16S rRNA Gene Sequencing 2.5 Data Analysis 3 Results 4 Discussion 5 Conclusion References The Role of Cell-Free RNA in Clinical Diagnosis and Evaluation of Cell-Free RNA Extraction Methods 1 Introduction 2 Review Method 3 Cell-Free RNA in Clinical Diagnosis 3.1 Cell-Free RNA in Cancer Diagnosis 3.2 Cell-Free RNA in Neurodegenerative Diseases Diagnosis 4 Extraction of cf-RNA 4.1 Extraction Methods 4.2 Evaluation Criteria and Methods 5 Discussion and Conclusion References Study on Extracting Crude Phycocyanin from Spirulina Algae and Determining Its Ability in Protecting Fibroblasts from Oxidative Stress of Hydroxyl Peroxide 1 Introduction 2 Materials and Methods 2.1 Materials and Reagents 2.2 Extraction and Purification of C-Phycocyanin 2.3 Evaluation of Cytotoxicity of CrPC In Vitro [11] 2.4 Evaluation of the Proliferation Effect of CrPC [12] 2.5 Evaluation of the Migration Effect of CrPC by Wound Healing Assay [13] 2.6 Evaluation of the Protective Ability of CrPC Against H2O2 2.7 Statistical Analysis 3 Result 3.1 Extraction of CrPC 3.2 Evaluation of Cytotoxicity of CrPC 3.3 Evaluation of Proliferation Effect of CrPC 3.4 Evaluation of Migration of CrPC 3.5 Evaluation of the Protective Ability of C-PC Against H2O2 4 Discussion and Conclusion 5 Conclusion References Isolation of Antimicrobial Probiotic Bacteria from Sour Shrimps in Hue City-Vietnam and Optimization for Biomass and Acid Production 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 Isolation of Potential Probiotics Strains 2.3 Primary Screening for Potential Probiotics Strain 2.4 Screening for Probiotics Strains Strongly Inhibiting Indicator Bacteria 2.5 Agar-Well Diffusion Assay 2.6 Dual Culture Overlay Assay 2.7 Molecular Identification of Potential Strain 2.8 Optimization of Culture Conditions for V101 Growth and Total Acid Production 2.9 Growth and Antimicrobial Activities of Probiotics Strain Under Optimal Culture Conditions 2.10 Building the Biomass Standard Curve of V101 Strain 2.11 Quantitative Determination of Total Acid Concentration by Titration 2.12 Data Analysis 3 Results and Discussions 3.1 Isolation Potential Probiotics Strains 3.2 Colony Morphological Analysis and Sequencing 16S rRNA of V101 Strain 3.3 Biomass Standard Curve 3.4 Optimization of Appropriate Culturing Conditions for Bacterial Growth and Total Acid Accumulation 3.5 Culturing V101 Under Optimum Conditions 3.6 Pilot Fermentation 4 Conclusions References Investigation Fermentation of Oolong for Stress Suppressing Drink and Enriching GABA—A Neurological Nourishing Compound 1 Introduction 2 Materials and Methods 2.1 Materials 2.2 GABA Enriching Fermentation of Oolong Tea 2.3 GABA Enriching Bulk Fermentation of Oolong Tea Supplied with Viscozyme L 2.4 GABA Enriching Bulk Fermentation of Oolong Tea Inoculated with L. brevis 2.5 Determination of Tannin Content 2.6 Determination of Total Color (TC) 2.7 Determination of Thearubigins (TR) and Theaflavin (TF) 2.8 Determination of GABA Content 2.9 Sensory Assessment 2.10 Data Analysis 3 Results and Discussions 3.1 Quality of Oolong Tea Sample Prepared for Fermentation 3.2 Investigate the Fluctuation of Physical and Chemical Components of Oolong Tea in the Bulk Fermentation 3.3 Investigate the Fluctuation of Physical and Chemical Components of Oolong Tea in Fermentation with Enzyme 3.4 Investigate the Fluctuation of Physical and Chemical Components of Oolong Tea in Fermentation with L. brevis 3.5 Sensory Assessment the Oolong Fermented with L. brevis 4 Conclusions References Enhanced Activation of Integrin αIIbβ3-Dependent Signaling in the Pro33 (HPA-1b) Variant 1 Introduction 2 Materials and Methods 3 Results 4 Discussion References AI and Data Science for Health Object Contour in Medical Images Based on Saliency Map Combined with Active Contour Model 1 Introduction 2 Salient Map and Active Contour Model 2.1 Salient Map 2.2 Active Contour Model 3 Contour Detection Based on Active Contour Model in Salient Regions 3.1 Improving the Region’s Quality of Objects 3.2 Superpixels for Regions 3.3 Salient Map for Medical Images 3.4 Active Contour Model in Salient Regions 4 Experiments and Results 5 Conclusions References Visual Object Tracking Method of Spatio-temporal Context Learning with Scale Variation 1 Introduction 2 Methods of Visual Tracking 2.1 Spatio-Temporal Context Algorithm 2.2 Size Variation Problem and Solution 3 Results and Discussions 4 Conclusions References Weighted Box Fusion Ensembling for Lung Disease Detection 1 Introduction 2 Proposed Method 2.1 Dataset 2.2 Weighted Boxes Fusion 2.3 Weighted Boxes Fusion Algorithm Description 3 Results and Discussions 3.1 The Effectiveness of WBF Over Baseline 3.2 The Effectiveness of WBF When Comparing with Different Approaches 4 Conclusion References Epileptic Seizure Detection Based on Electroencephalography Signals and One-Dimensional Convolutional Neural Network 1 Introduction 2 Related Work 3 Method 3.1 Dataset 3.2 Convolutional Neural Network Architecture 4 Result 4.1 Training Efficiency 4.2 Prediction Result 5 Discussion 6 Conclusion References Classification of Four-Class Motor-Imagery Data for Brain-Computer Interfaces 1 Introduction 2 Materials and Methods 2.1 Data Acquisition and Dataset 2.2 Preprocessing 2.3 Feature Extraction 2.4 Feature Selection 2.5 Classification 3 Evaluation Metric 4 Result and Discussion 5 Conclusion References Segmentation of Brain Tumour Using UNET Architecture 1 Introduction 1.1 A Subsection Sample 2 Method 2.1 Database 2.2 Pre-processing 2.3 MultiResUNet 2.4 Post-processing 2.5 Evaluation Metric 3 Results and Discussion 4 Conclusion References Deep Learning-Based Automatic Detection of Defective Tablets in Pharmaceutical Manufacturing 1 Introduction 2 Material and Methods 2.1 The Overall Framework for Intelligent Visual Inspection 2.2 Data Acquisition, Image Preprocessing and Feature Extraction 2.3 Deep Learning Models 2.4 Training Strategy and Experiment Setup 2.5 Performance Evaluation 3 Classification Results 4 Discussion and Conclusion 4.1 Comparison with the Previous Studies 4.2 Limitations and Future Works References Adaptive Lung Diseases Images Classification Technique Based on Deep Learning 1 Introduction 2 Background of Deep Learning for Classification 3 Lung Diseases Image Classification 4 Experiments and Results 4.1 The Dataset for Training and Testing 4.2 Experiments 5 Conclusions References A Machine Learning Framework Based on Extreme Gradient Boosting for Intelligent Alzheimer's Disease Diagnosis Using Structure MRI 1 Introduction 2 Materials and Methods 2.1 Data Acquisition 2.2 Overall Framework 2.3 Preprocessing of Structural MRI and Feature Extraction 2.4 T-Test-Based Feature Selection and Statistical Analysis 2.5 Machine Learning Classifier 2.6 Performance Evaluation 3 Results 3.1 Preprocessing Results 3.2 Classification Results 3.3 Affected Cortical Regions (Feature Ranking and Feature-Based Classification Results) 4 Discussion 5 Conclusion References Automatic White Blood Cell Classification Using Deep Learning Network 1 Introduction 2 Methodology 2.1 Dataset 2.2 Experimental Framework 3 Result and Discussion 4 Conclusion References Automatic Foveal Avascular Zone Segmentation Using Hessian-Based Filter and U-Net Deep Learning Network 1 Introduction 2 Method 2.1 Dataset 2.2 Hessian Filter 2.3 U-Net Deep Learning Network 2.4 Level Set 2.5 Jaccard Coefficient: 3 Results 4 Conclusion and Discussion References Healthcare Informatics and Public Health Ascorbic Acid as a Radiation-Protective Agent Against Ionizing Radiation 1 Introduction 2 Materials and Methods 2.1 Samples 2.2 Irradiation 2.3 Analysis of Strand Breaks 2.4 Survival Rate (SR) 3 Results 3.1 Protective Effect of AA on DNA 3.2 Protective Effect of AA on Living Cells 4 Discussion 5 Conclusion References The Effectiveness Validation of Novel UVC Air Disinfection Device in a Hospital Room Using Computational Fluid Dynamic (CFD) Simulation 1 Introduction 2 Materials and Method 2.1 Computational Fluid Dynamics (CFD) Modeling Method 2.2 Airborne